The nine C-terminal residues of the grapevine fanleaf nepovirus movement protein are critical for systemic virus spread.
نویسندگان
چکیده
منابع مشابه
Isolation of the Gene Coding for Movement Protein from Grapevine Fanleaf Virus
A pair of degenerate primers, GMPF1 and GMPR1, was designed on the basis of alignment of previously reported Grapevine fanleaf virus (GFLV) movement protein (MP) nucleotide sequences from Iran and other parts of the world. cDNA was synthesized by the use of Oligo d(T)18 from total RNA extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (PCR) with the d...
متن کاملisolation of the gene coding for movement protein from grapevine fanleaf virus
a pair of degenerate primers, gmpf1 and gmpr1, was designed on the basis of alignment of previously reported grapevine fanleaf virus (gflv) movement protein (mp) nucleotide sequences from iran and other parts of the world. cdna was synthesized by the use of oligo d(t)18 from total rna extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (pcr) with the d...
متن کاملGrapevine fanleaf nepovirus P38 putative movement protein is not transiently expressed and is a stable final maturation product in vivo.
The putative 38 kDa movement protein (P38) gene, located on the RNA2 of grapevine fanleaf nepovirus (GFLV), was cloned in Escherichia coli and expressed as a fusion protein fused to six histidines (6HisP38). This protein was purified and used to produce a specific polyclonal antiserum from which immunoglobulins were isolated by immunoaffinity against the recombinant 6HisP38. Western immunoblot ...
متن کاملCharacterization of the Full Length Coat Protein Gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis
The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population ...
متن کاملProduction of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli
The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved wi...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1999
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-80-6-1347